3
rd
International Conference on
Biomarkers & Clinical Research
July 2-4, 2012 Embassy Suites Las Vegas, USA
July 2-4, 2012
Volume 3 Issue 4 - 26
J Mol Biomark Diagn
ISSN:2155-9929 JMBD an open access journal
Hitoshi Sohma
Sapporo Medical University Center for Medical Education, Japan
Hitoshi Sohma, J Mol Biomark Diagn, 3:4
http://dx.doi.org/10.4172/2155-9929.S1.008
Investigation of annexin A5 as a biomarker for Alzheimer’s
disease and Dementia with Lewy bodies
Hitoshi Sohma
1,2
, Shin-ichi Imai
2
, Kayo Matsuki
2
, Norio Takei
2
, Hirohito Honda
3
, Kumiko Utsumi
4
, Eri Hashimoto
5
,
Toshikazu Saito
5
and Yasuo Kokai
2
1
Department of Educational Development, Sapporo Medical University Center for Medical Education, Sapporo, Japan
2
Department of Biomedical Engineering, Sapporo Medical University School of Medicine, Sapporo, Japan
3
Sanyo Chemical Industries, Ltd., Kyoto, Japan
4
Neuropsychiatry, Sunagawa City Medical Center, Sunagawa, Japan
5
Department of Neuropsychiatry, Sapporo Medical University School of Medicine, Sapporo, Japan
Background: Alzheimer’s disease (AD) diers from other forms of dementia in its relation to amyloid beta peptide
(A☐42). Using a cell culture model we previously identied annexin A5, a Ca2+ and phospholipid binding
protein, as an AD biomarker. Plasma level of annexin A5 was signicantly higher in AD patients compared to that
in a control group. As AD shares clinical and pathological features with Dementia with Lewy bodies (DLB), the
present study was done to examine the similarity of AD and DLB using the plasma AD biomarker annexin A5.
Methods: e manner in which this study was conducted met with the approval of Sapporo Medical University
Ethics Committee. Blood samples were obtained from 150 patients with AD (aged 77.6 ± 6.5 years), 50 patients of
DLB (79.4 ± 5.0) and 279 community-dwelling healthy elderly individuals of comparable age and sex (75.6 ± 8.1).
All AD patients met NINCDS-ADRDA criteria and all DLB patients were diagnosed as probable DLB according to
the latest consensus diagnostic criteria. Blood was drawn with Venoject II vacuum tubes containing EDTA and the
plasma fraction was isolated by centrifugation. Annexin A5 present in the specimen was trapped by a monoclonal
antibody (mAb) against annexin A5 (clone No. 23) conjugated to a glass bead, and an HRP-labeled mAb against
annexin A5 (No. 49). Quantication was done using the Chemiluminescent Enzyme Immunoassay Technique
(SphereLight assay). is system was useful to quantify plasma annexin A5 from the rage of 0.16-20ng/ml.
Results: e plasma level of annexin A5 was signicantly higher in AD patients than in the healthy individuals
(control) (P < 0.0001). e plasma annexin A5 level was also signicantly higher in DLB patients than in the
control group (P < 0.0001). From the ROC curve with plasma annexin A5 concentrations, the mean areas
under the curve were 0.898 and 0.838 for the AD/control and DLB/control, respectively. is suggests that
annexin A5 is a common marker for both AD and DLB, which implies the probability of a pathological aspect
of overlapping of AD and DLB.
Conclusions: Annexin A5 is a novel plasma biomarker candidate for both AD and DLB.
Biography
Hitoshi Sohma completed his Ph.D. in biochemistry at Hokkaido University, Japan, focusing on Ca2+-signaling in cell-cell
communications, and his postdoctoral studies at the National Institute of Mental Health, NIH. He is a professor in the Department
of Educational Development, Sapporo Medical University Center for Medical Education, Sapporo, Japan. He is also involved in
both pathobiochemical research and the management of medical education at the university.
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